Primer Design Tools
autoprime
http://www.autoprime.de/
bioweb Primer Design
http://bioweb.uwlax.edu/GenWeb/Molecular/Seq_Anal/Primer_Design/primer_design.htm
GeneFisher2
http://bibiserv.techfak.uni-bielefeld.de/genefisher2/submission.html
MethPrimer
http://www.urogene.org/methprimer/
OligoPicker
http://pga.mgh.harvard.edu/oligopicker/index.html
OligoWiz 2 Server
http://www.cbs.dtu.dk/services/OligoWiz/
Online Analysis Tools - PCR
http://molbiol-tools.ca/PCR.htm
PCR Primer Stats
http://www.bioinformatics.org/sms2/pcr_primer_stats.html
PDA
http://dbb.nhri.org.tw/primer/
PerlPrimer
http://perlprimer.sourceforge.net/
Pride
http://pride.molgen.mpg.de/pride.html
Primer Blast NCBI
http://www.ncbi.nlm.nih.gov/tools/primer-blast/
Primer design - Oligo (50mer - 70mer) design software (for DNA chips and sequencing)
http://pride.molgen.mpg.de/
Primer search
http://www.genomatix.de/online_help/help_gems/pod_help.html
Primer3
http://bioinfo.ut.ee/primer3-0.4.0/
Primer3
http://biotools.umassmed.edu/bioapps/primer3_www.cgi
primerbank
http://pga.mgh.harvard.edu/primerbank/
PrimerPremier
http://www.premierbiosoft.com/netprimer/index.html
PrimerStation
http://ps.cb.k.u-tokyo.ac.jp/index.html
PrimerX
http://bioinformatics.org/primerx/
primerz streamlined primer design for promoters
http://grch37.genepipe.ncgm.sinica.edu.tw/primerz/beginDesign.do
primo
http://www.changbioscience.com/primo/primo.html
Probe Design using OligoWiz 2.0
https://services.healthtech.dtu.dk/
ProbeWiz Server
http://www.cbs.dtu.dk/services/DNAarray/probewiz.html
ProMide
http://oligos.molgen.mpg.de/
RepeatMasker
http://www.repeatmasker.org/
UCSC In-Silico PCR
http://genome.ucsc.edu/cgi-bin/hgPcr
Visual Gene Developer
http://www.visualgenedeveloper.net/